Figure 4
![Figure 4. Warfarin prevents the initiation of an immune response to FVIII. FVIII−/−/BALB/c mice were pretreated with warfarin (n = 6) or PBS (n = 7) before the first and third of 5 intraperitoneal injections of 1 μg FVIII. This experiment was repeated twice, and representative data are shown. Seven days after the last injection, (A) serum samples were analyzed for antibody response (582 ± 232 [FVIII] vs 101 ± 24 μg/mL [FVIII + warfarin]; *P = .042) and (B) splenic T cells from the mice treated with FVIII or FVIII + warfarin were assayed for the response to FVIII (**P = .002). These data indicate that other coagulation factors besides FVIII are probably necessary for initiation of the immune response.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/114/21/10.1182_blood-2008-10-186452/4/zh89990944920004.jpeg?Expires=1724275616&Signature=mqORd5Hkw-uz65ffEnXRahMxgHxx2FHDdQMMrJ-ZR~3WTUhPBaEwaCxCcJX~fA9T62GuZkDHmYt3mskReH~7wtrAIn75qspIW4TFJqsO-6nDczMqtPmoh720~R86CqNmlnwBD4VAp2I~ZiY6tc3k8t74A~heR1DA3Qg4BbzTC6pR2o6M~u4Mse75ti8PM7hGcHuFw~51I1-HcCePzKVpinkV6CNUlQZs43KytHrMEVx3r0S3Cs9fYquO4PXAg7AZxvdCYfji4MAgi3nG5391ioLDVyKaubhDxQ8dJ5oY5YImK6BkbvS0oTLVMJwbWDq78xOLnFa3d71h6xeA~fEFnQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Warfarin prevents the initiation of an immune response to FVIII. FVIII−/−/BALB/c mice were pretreated with warfarin (n = 6) or PBS (n = 7) before the first and third of 5 intraperitoneal injections of 1 μg FVIII. This experiment was repeated twice, and representative data are shown. Seven days after the last injection, (A) serum samples were analyzed for antibody response (582 ± 232 [FVIII] vs 101 ± 24 μg/mL [FVIII + warfarin]; *P = .042) and (B) splenic T cells from the mice treated with FVIII or FVIII + warfarin were assayed for the response to FVIII (**P = .002). These data indicate that other coagulation factors besides FVIII are probably necessary for initiation of the immune response.
