Figure 4
Induction of active CSR in patients with CVID or IgAD. Expression of AID mRNA, rate of Iγ-Cγ or Iα-Cα germline transcription, and presence of Iγ-Cμ or Iα-Cμ switch circle transcripts in PBMCs from 15 patients with CVID (A-B) and 10 subjects with IgAD (C) at day 3 of cell culture with IL-21 (10 ng/mL), IL-4 (0.5 ng/mL), and anti–human CD40 mAb (2 μg/mL). The diameter of the dots correlates proportionally with units of secreted IgG (A) or IgA (B-C), detected in 20 hours of ELISPOT assay at day 5 of culture using 5 × 104 PBMCs.
