NF-κB inhibitor Bay 11-7082 reverses the expression of CCL20 and TNF-α. Confluent monolayers of HBMECs were pretreated with indicated concentrations of Bay 11-7082 for 1 hour, followed by 6 hours of coincubation with Pf-IRBCs. Protein levels of CCL20 (A) and TNF-α (B) were assayed in culture supernatants by ELISA. Data are presented as mean ± SD of duplicate experiments with 3 biologic replicates (n = 6).