Figure 6
The kinetics of p65 NF-κB nuclear translocation of Pf-IRBC–activated HBMECs and inhibition by IκB phosphorylation inhibitor Bay 11-7082. Confluent HBMECs were incubated in the absence or presence of Pf-IRBCs (5 × 108/100 × 20-mm culture dishes) for the times indicated. In addition, cells were also pretreated with Bay 11-7082 for 1 hour where indicated. Cytosolic and nuclear proteins (25 μg) were resolved on sodium dodecyl sulfate–polyacrylamide gel electrophoresis and Western blotting was performed.
