NF-κB signaling pathway was the most significant canonical pathway in HBMEC up-regulation. Genes, differentially regulated in HBMECs in response to Pf-IRBC coincubation, were clustered into canonical pathways using Ingenuity tool (Ingenuity Systems). The NF-κB pathway was statistically most significant (P = .001). Colored genes were up-regulated in the presence of Pf-IRBC.