Figure 3
Figure 3. Effect of chelating agents on [Ca2+]i elevations in platelets interacting with acid-soluble type I collagen under flow. Blood cell suspensions (Figure 1 legend contains details) were perfused over collagen at the shear rate of 250 seconds without or with prior treatment with a Ca2+ chelator, the membrane impermeable EGTA (5 mM), the membrane permeable BAPTA-AM (80 μM), or the 2 combined. Results are expressed as described in the legend to Figure 2 (n = 3).

Effect of chelating agents on [Ca2+]i elevations in platelets interacting with acid-soluble type I collagen under flow. Blood cell suspensions (Figure 1 legend contains details) were perfused over collagen at the shear rate of 250 seconds without or with prior treatment with a Ca2+ chelator, the membrane impermeable EGTA (5 mM), the membrane permeable BAPTA-AM (80 μM), or the 2 combined. Results are expressed as described in the legend to Figure 2 (n = 3).

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