Inhibition of nuclear translocation of CD43ct induces apoptosis. (A) Diagram of the plasmid encoding the ER-CD43ct fusion protein. (B) CD43-deficient bone marrow cells infected with ER-CD43ct or ER were grown for 5 days in IL-3/G-CSF. Percentages of GFP⁺/PI⁻ cells are shown in cultures supplemented with increasing concentrations of tamoxifen. (C) Aliquots of cultures kept in 100 nM tamoxifen were deprived of tamoxifen at day 5 for 1 day. Percentages of PI⁺ and percentages of annexin V⁺ cells are shown at day 6 in cultures maintained without tamoxifen and cultures with tamoxifen. **P = .001. Data are mean ± SD (n = 3) and are representative of 2 independent experiments.