CD43ct is localized in the nucleus. (A) Fluorescent microscopy of CD43-deficient bone marrow cells infected with CD43-GFP. DAPI (4,6 diamidino-2-phenylindole) staining shows the location of the nucleus. (B) Fluorescent microscopy of CD43-deficient bone marrow cells infected with GFP alone as a control. (C) Fluorescent microscopy of CD43-deficient macrophages infected with CD43-GFP. (D) Confocal microscopy of total bone marrow cells stained with S11 and H18 antibodies (top panel) and confocal microscopy of mitogen-activated T cells stained with H18 antibody and Topro-3 nuclear stain (bottom panel).