Recognition of other leukemic cells. Acute myeloid leukemia (AML) cells (CD33⁺/CD14⁻) from 3 patients with known HLA and mHag typing were isolated from PBMCs (PBZ, SBC) or bone marrow mononuclear cells (SPA) by flow cytometry. The isolated AML cells were tested for recognition by T-cell clone 62 (A), clone 78 (B), clone 80 (C), and clone 87 (D). EBV-LCLs of donor and patient served as negative and positive control, respectively. The SNP genotyping data (mHag⁺ or mHag⁻) and expression of the respective HLA-DR restriction molecule (DR⁺ or DR⁻) are indicated for each of the 4 mHags. All 4 T-cell clones recognized mHag⁺ AML cells expressing the appropriate HLA-DR restriction molecules. Mean release of IFN-γ (ng/mL) in 50-μL supernatants of duplicate wells is shown. nd indicates not determined.