Effect of RAP on up-regulation of MMP-3 expression and increased ICB induced by t-PA treatment after MCA occlusion in mice. Immunohistochemical analysis of MMP-3 and CD31 expression (A-F). MMP-3 immunoreactivity (green: fluorescein) colocalizing with CD31 immunoreactivity (red: rodamine) at the border zone of the damaged brain after t-PA treatment without (A-C) or with (D-F) RAP pretreatment. The arrows represent endothelial cells, and the scale bar represents 50 μm. Representative pictures are shown of a damaged brain section after MCA-O without t-PA treatment (G), with t-PA treatment (H), with t-PA and 1 mg/kg RAP (I), or with t-PA and 2 mg/kg RAP (J). (K) Quantitative data are also shown. Data represent mean ± SD of 8 experiments. *P < .05; **P < .01.