Findings of granulomatous colitis and partial reduction in neutrophil oxidase activity as measured by DHR123 assay. (A) Diffuse aphthous ulcerations (
) were visible throughout the colon at the time of presentation. Biopsies showed prominent epithelioid granulomata in lymphoid aggregates (B,
) and in lamina propria (C) with giant cells (inset). Four-μm sections were stained using hematoxylin and eosin prior to microscopy and photomicrographs were taken with a Nikon Eclipse 80i microscope equipped with a Nikon Digital Sight DS-U1 camera using 20×/0.50NA and 40×/0.75NA objectives. (D) DHR123 fluorescence in control (black line) and patient (red line) neutrophils before and after stimulation with 0.6 μg/mL PMA (representative of 5 assays).