H/F-LV transduction of quiescent lymphocytes induces high levels of reverse-transcribed viral DNA. (A) Quiescent B and T cells were transduced with H/F and VSVG pseudotyped LVs at an MOI of 10 and 30, respectively. A time-course assay was performed to determine total-LTR content per cell and 2-LTR circles per cell present in VSVG-LV– and H/F-LV–transduced quiescent B cells at 10 minutes, 12 hours, 48 hours, and 12 days after transduction. The specific primers used are described in “DNA extraction and Q-PCR.” The total-LTR copies per cell and number of 2-LTR circles per cell are indicated in the y-axis (mean ± SD; n = 3). (B) Time-course assay for transduction of quiescent T cells was performed as in panel A. The total-LTR copies per cell and number of 2-LTR circles per cell are indicated in the y-axis (mean ± SD; n = 3). (C) Quiescent adult B cells were transduced with H/F-LVs (MOI = 10) or VSVG-LVs (MOI = 30) in the absence or presence of AZT. Total-LTR copies per cell present in VSVG-LV– and H/F-LV–transduced quiescent B cells at 12 and 24 hours is shown. The number of total-LTR copies per cell is indicated in the y-axis (mean ± SD; n = 3). (D) Time-course assay for transduction of quiescent T cells was performed as in panel C.