Abundance (RPKM) measured by RNA-seq in platelets correlates to real-time PCR results and is reproducible between independent platelet isolations. (A-B) Scatter plots comparing RNA-seq–derived RPKM measurements with real-time PCR results from independently isolated platelet preparations from (A) human and (B) mouse. The adjusted 2^−Ct value for each gene as measured by real-time PCR are plotted along the x-axis versus the same gene's RPKM on the y-axis. (C-D) Scatter plots demonstrate the within-species correlation of RPKM measurements between independent samples. (C) Human platelet sample split and stimulated with thrombin (y-axis) or left unstimulated (x-axis) before RNA isolation and sequencing. (D) Comparison of RPKM measurements from 2 independent mouse platelet isolations. ρ indicates the Spearman rank correlation coefficient.