Effector memory CD8⁺ T cells isolated ex vivo and IL-12 programmed effector CD8⁺ T cells generated in vitro share a set of commonly regulated genes. Differentially expressed genes from the ex vivo samples were reassessed to identify genes expressed > 1.7-fold higher in all donors within the CXCR3^hi compared with the CCR7^hi cells. These genes were then assessed for commonality with the induced genes identified in cells activated with IL-12 and IL-12 + IFN-α culture conditions. (A) Genes up-regulated by either IL-12 or IL-12 + IFN-α and also increased in CD8⁺CCR7^loCXCR3^hi cells are shown in the Venn diagram. (B-C) Normalized expression values of selected genes are plotted as box-whisker bars (whiskers represent range).