CCR7^loCXCR3^hi effector memory CD8⁺ T cells secrete effector cytokines. CCR7^hiCXCR3^lo, CCR7^hiCXCR3^hi, and CCR7^loCXCR3^hi CD8⁺ T cells were sorted from healthy human PBMCs and labeled with CFSE. The cells were stimulated with plate-bound anti-CD3 for 3 days in the absence or presence of 200 U/mL of rhIL-2. The cells were restimulated with phorbol myristate acetate and ionomycin in the presence of brefeldin A for 4 hours on day 3 after stimulation. Proliferation was measured by CFSE dilution, and IFN-γ was measured by intracellular staining. (B) The percentage of cells positive for IFN-γ expression as a function of cell division are plotted as mean ± SEM for the cells receiving exogenous IL-2 as indicated by the gates in panel A (far right dot plots). IFN-γ (C) and TNF-α (D) were measured in the supernatants by ELISA.