TSLP induces redistribution and colocalization of actin and phospho-myosin II light chain. (A) After 24 hours of culture, TSLP-DCs were stained for myosin II (green), actin (red), and DAPI (blue). TSLP-DCs acquired a polarized morphology, and actin and myosin filaments largely colocalized. Data are from 1 representative of 3 independent experiments. (B) DCs on poly-lysine–coated coverslips were cultured in medium, TSLP, or LPS for 20 hours. Cells were stained for F-actin (red) and pMLC9 (green). Images were acquired as z-series, deconvoluted, and reconstructed as a maximum-intensity projection of all the planes. (C) Quantification of the mean percentage of pMLC area colocalized with F-actin area. For each cell, the percentage is a mean of all the planes. Data are mean ± SEM of 2 independent experiments; n = 20. ***P < .005.