TSLP induces chemokinesis of resting DCs in a cell-autonomous manner. (A) Purified blood DCs were precultured in medium (untreated), TSLP, influenza virus (Flu), LPS, or GM-CSF. After 24 hours, cells were washed, counted, and seeded in equal numbers in the upper chamber of an uncoated transwell system in the absence of chemotactic factors. After 6 hours, DCs migrating into the lower chamber were harvested and counted. Data are represented as percentage of input DCs. In the positive control, CCL20 was used in the lower chamber during migration as a chemotactic factor. Data are mean ± SD; n = 7. *P < .05 vs untreated. (B) Primary blood DCs were precultured in medium, TSLP, TNF, influenza virus (Flu), LPS, or GM-CSF. After 24 hours, cells were washed, counted, and seeded in equal numbers in the upper chamber of a collagen-coated transwell system in the absence of chemotactic factors. After 6 hours, DC migration was quantified. Data are represented as percentage of input DCs. In the positive control, CCL20 was added in the lower well. Data are mean ± SD; n = 5. *P < .05 vs untreated.