CBS9106 reduces CRM1. Immunoblot (left) of MM.1S cells and the relative expression level of CRM1 (right). (A) MM cells were treated with vehicle or CBS9106 (1-400nM) for 24 hours, and the whole-cell lysates were analyzed by immunoblotting for the presence of indicated proteins with corresponding antibodies. Expression levels of CRM1 protein in 4 MM cell lines were normalized relative to a loading control band of actin and are expressed as ratios relative to the corresponding control. (B) MM.1S cells were treated with CBS9106 (100nM) for 0-24 hours, and the whole-cell lysates were analyzed by immunoblotting (left). Expression levels of CRM1 protein were normalized with a loading control band of actin and are expressed as ratios relative to the corresponding control (right).