LOXL2 is involved in endothelial migration and proliferation. (A-B) HUVEC motility on fibronectin in serum-free medium containing 10 ng/mL VEGF and 10 ng/mL bFGF was measured by time-lapse microscopy. Images were acquired every 5 minutes over the next 12 hours for cell tracking. For each experiment (A: n = 4; B: n = 3), velocity was normalized to the mean of control cell velocity. Graph represents the mean of normalized values ± SEM. (A) HUVECs were transfected with either control (sictl) or loxl2 (siloxl2) siRNA. (B) HUVEC were transfected with a plasmid either empty (ctl) or coding for LOXL2 and incubated in the presence or absence of β-APN. (C) HUVEC migration in 3D fibrin gel was assessed using cells infected with control (ctl) or LOXL2 coding lentivirus. HUVEC-coated Cytodex beads were embedded in a fibrin gel and maintained in complete medium supplemented with 10 ng/mL bFGF. Brightfield images were acquired (left panel) for migration measurement (right panel). Mean distance per bead was normalized to the mean of controls. Bar: 200 μm. (D) FBS-induced proliferation of HUVECs infected with lentiviruses coding for either control (shctl) or lox (shlox) or loxl2 (shloxl2) shRNA. Graph represents the mean of normalized values ± SD. ***P < .001.