Figure 7
Innate lymphocyte IFN-γ response induced by zoledronate depends on endogenous caspase-1. (A) CD56+ PBMCs (1.5 × 106/mL) were stimulated for 20 hours with either IL-2 alone (100 U/mL) or with zoledronate (30μM) plus IL-2 in the presence or absence of the caspase-1 inhibitor YVAD at 30μM (or DMSO solvent control). Cells were stained for intracellular IFN-γ and for surface CD3. (B) IFN-γ levels were determined in culture supernatants. One of 3 independent experiments is shown.
