Effects of ASO-21 and ASO-155 in KAI3 cells. (A) Northern analysis of miR-21 and miR-155 in KAI3 cells treated with both ASO-21 and ASO-155 and in cells treated with SCO-21 and SCO-155. (B) Western analysis of PTEN and SHIP1 and their downstream targets. Fold changes in protein levels are shown below the gels and are normalized to the level in SCO-treated cells, which was assigned a value of 1.00. (C) Incidence of apoptosis among KAI3 cells. Cells treated with both ASOs or SCOs were exposed to 50 μmol/L etoposide for 2 hours. (Top panels) Flow cytometric analysis is shown. y-axis: number of cells stained with PI; x-axis: cells stained with annexin V–FITC. Apoptotic cells are in R1 and R2; necrotic cells are in R3. (Bottom panel) □ and ■ indicate percentage of apoptosis (R1 + R2) or necrosis (R3) among SCO-21/155–treated and ASO-21/155–treated cells, respectively. Symbols and bars indicate means and SDs of triplicate samples. Both apoptotic (R1 + R2) and necrotic (R3) cells of ASO-21/155–treated cells show significantly greater percentages than those cells treated with SCO-21/155.