miR-155 induces dysregulation of SHIP1 signaling to AKT in NK-cell lymphoma/leukemia lines. (A) Northern analysis of miR-155 in SNK6 and MOTN1 cells treated with ASO-155 or SCO-155. Controls (5S tRNA) are shown below the miR-155 blots. (B) Western analysis of PTEN, pAKT, total AKT, p21, p27, and p53 expression in MOTN1 and SNK6 cells treated with ASO-155 or SCO-155. Fold changes in protein expressions are shown below the gels and are normalized to the levels in SCO-treated SNK6 or MOTN1 cells, respectively, which were assigned a value of 1.00. (C) Western analysis of SHIP1, total AKT, pAKT, and phospho-NFκB (Phospho p65) in DERL7 cells transduced with miR-155 plus GFP or GFP alone (control). Fold changes in protein expression are shown below the gels and are normalized to the level in the GFP-transduced control cells, which was assigned a value of 1.00. (D) Luciferase reporter assay of INPP5D/SHIP expression in Rat-1 cells transfected with miR-155 (PMXs-miR-155-puro) or empty vector (PMXs-puro). Blots showing MiR-155 expression are beside the bars. The significance of differences between wild-type pGL3-INPP5D 3′UTR and pGL3-INPP5D was evaluated with the Student t test.