Figure 3
Quantitative PCR analysis of miRNA levels in specimens of primary NK-cell lymphoma/leukemia. (A) Histopathologic features of a typical specimen of primary extranodal NK/T-cell lymphoma, nasal type (NKpt5). Hematoxylin and eosin–stained (×40), CD56-stained, EBER-ISH–stained, and TIA-1-stained (×200) cells are shown. (B) Quantitative PCR analysis of mature miR-21, miR-23a, miR-29a, and miR-155 in normal sCD3−CD56+ cells (n = 8), NK-cell lymphoma/leukemia lines (n = 10), and specimens of primary NK-cell lymphoma/leukemia (n = 10). Y-axis: −ΔΔCt values for miRNA expression. The significance of differences between groups was evaluated by the use of the Student t test. (C) Correlation between miR-21 and miR-155 expression in 10 primary lymphoma/leukemia specimens. y-axis, −ΔΔCt values of miR-21 expression; x-axis, −ΔΔCt values miR-155 expression. The P value was calculated by simple regression analysis.

Quantitative PCR analysis of miRNA levels in specimens of primary NK-cell lymphoma/leukemia. (A) Histopathologic features of a typical specimen of primary extranodal NK/T-cell lymphoma, nasal type (NKpt5). Hematoxylin and eosin–stained (×40), CD56-stained, EBER-ISH–stained, and TIA-1-stained (×200) cells are shown. (B) Quantitative PCR analysis of mature miR-21, miR-23a, miR-29a, and miR-155 in normal sCD3−CD56+ cells (n = 8), NK-cell lymphoma/leukemia lines (n = 10), and specimens of primary NK-cell lymphoma/leukemia (n = 10). Y-axis: −ΔΔCt values for miRNA expression. The significance of differences between groups was evaluated by the use of the Student t test. (C) Correlation between miR-21 and miR-155 expression in 10 primary lymphoma/leukemia specimens. y-axis, −ΔΔCt values of miR-21 expression; x-axis, −ΔΔCt values miR-155 expression. The P value was calculated by simple regression analysis.

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