Comparison of miRNA expression in T-, B-, and NK-cell lymphoma/leukemia lines with quantitative RQ-PCR (Taqman PCR). (A) Taqman PCR analysis of miR-21, miR-23a, miR-29a, and miR-155 expression in 4 T-cell, 10 NK-cell, and 14 B-cell lymphoma/leukemia lines. The significance of differences between T- and NK-cell, between NK- and B-cell, and between T- and B-cell lymphoma/leukemia lines were assessed by use of the Student t test. The following B- and T-cell lymphoma/leukemia lines were used for this study. SUDHL4, SUDHL6, OCI-Ly3, and OCI-Ly7 cells were derived from diffuse large B-cell lymphoma; Raji, Daudi, Ramous, Namalwa, and D.G-75 cells were derived from Burkitt lymphoma; SP-49 and Jeko-1 cells were derived from mantle cell lymphoma; FL-18 cells were derived from follicular lymphoma; Karpass 1718 and SLVL cells were derived from splenic virus lymphoma; ATN1 cells were derived from adult T-cell leukemia; JM and MOLT4 cells were derived from T-cell leukemia; and MyLa cells were derived from peripheral T-cell lymphoma unspecified. (B) Correlation between expression miR-21 and miR-155 in 10 NK-cell lymphoma/leukemia lines. In the top panel, □ and ■ bars depict −ΔΔC_t values for miR-155 and miR-21 expression, respectively. In the bottom panel: y-axis, −ΔΔC_t values for miR-21 expression; x-axis, −ΔΔC_t values for miR-155 expression are shown. The P value was calculated by simple regression analysis.