Northern analysis of miR-21, miR-23a, miR-29a, and miR-155 in NK-cell lymphoma/leukemia lines. (A) Northern analysis of miR-21, miR-23a, miR-29a, and miR-155 in 10 NK-cell (CD3⁻CD19⁻CD56⁺; left panel), 2 T-cell (CD3⁺CD19⁻CD56⁻; right panel), and 7 B-cell (CD3⁻CD19⁺CD56⁻; right panel) lymphoma/leukemia lines. NKL and HANK1 are shown in both panels in duplicate to compare miRNA expressions. Fold changes in miRNA were determined by densitometry and are shown below the gels after normalization to the level of miR-21 in KAI3 cells, which was assigned a value of 1.00. The blots for all 4 membranes hybridized by γ³²-dATP-antisense oligo for microRNAs were exposed at the same condition (see “Methods”). (B) Expression of miR-21, miR-23a, miR-29a, and miR-155 measured in 3 samples of normal cells (n1, n2, and n3) expressing sCD3⁻CD56⁺. The level of miR-21 expression in KAI3 cells was assigned a value of 1.00. (C) Correlation between miR-21 and miR-155 expression. In the left panel, □ and ■ depict relative expression values for miR-155 and miR-21 expression, respectively. In the right panel, y-axis, expression values for miR-21 expression; x-axis, expression values for miR-155 expression. The P value was calculated by simple regression analysis.