Accelerated replication of PVM in mouse bmEos from MyD88^−/− mice. (A) Q-RT-PCR detection of virus titer in bmEos. Virus titer was determined in PVM-infected (filled symbols) and _hiPVM-challenged (open symbols) wild-type (C57BL/6; circles) and MyD88^−/− mouse bmEos (squares). Values shown are representative of 2 experiments performed in triplicate (data shown represent mean ± SEM); *P < .05. (B) Immunodetection of PVM in extracts of bmEos. PVM was detected at 9 days after inoculation of bmEos cultures on a Western blot probed with rabbit polyclonal anti-PVM N peptide antibody (αN_PVM). Lanes 1 to 3 are extracts from bmEos from wild-type C57BL/6 mice; lanes 4 to 6, extracts from bmEos from MyD88^−/− mice; lanes 1 and 4, from unchallenged bmEos (controls); lanes 2 and 5, from _hiPVM-challenged bmEos; lanes 3 and 6, from PVM-infected bmEos. After probing with anti-PVM N antibody the blot was stripped and probed with anti–mouse GAPDH (αGAPDH) as a control for protein loading. Blot shown is single membrane, divided to omit intervening protein marker lanes.