Immunophenotyping reveals that ME^m1/m1 mice lack long-term repopulating cells. (A) Analytical flow cytometry for immunophenotypic long-term repopulating cells (either CD150⁺/CD48⁻ or CD135⁻/CD34⁻) performed on ME^m1/m1 (KO) and WT BM cells as indicated. A representative experiment is shown; essentially identical results were obtained in 2 independent experiments. (B) Yield of Lin⁻ cells ± SD and LSKs ± SD (based on > 30 independent cohorts of mice). LT-LSKs, CMPs, CLPs, and GMPs are indicated; progenitor data are based on 3 independent experiments normalized to WT. Black bars indicate WT BM; gray bars, ME^m1/m1 BM.