Inflammatory signal screening. (A) RT-PCR array results of genes encoding common cytokines. The log10 of the cycle threshold (Ct) were compared in a scatter plot, where circles in the top left represent genes with higher expression in Gaucher disease MSCs and circles in the bottom right represent genes with higher expression in normal MSCs. Cytokines not detected in either sample are not shown. (B) RT-PCR array results of genes encoding inflammatory cytokines. (C) Autoradiogram of relevant portions of “Human Cytokine Antibody Array 8” membranes, which screen 172 cytokines and cytokine receptors. (D) Relative mRNA levels of various genes important for MSC interactions with surrounding cells, in Gaucher disease MSCs compared with normal MSCs. (E) Scanned autoradiogram of a Western blot performed for COX-2 expression analysis on passage 4 MSCs, with β-tubulin expression used as a loading control on the same samples. (F-H) Secretion levels of IL-8, PGE2, and CCL2 by MSCs of different passages. *P < .05. All error bars represent 1 SD.