Cholesterol content and lactosylceramide trafficking. (A) MSCs stained with filipin and observed under a fluorescence microscope; scale bar = 20 μm. (B) Total cholesterol and cholesteryl ester content expressed as nanograms per milligrams of total protein content in the samples. (C) Lysosomes (stained with Lyso Tracker Red) and BODIPY-lactosylceramide (green) after 2 hours of trafficking, with corresponding signal overlap in white, and correlation coefficients in the graph (Manders M1 corresponds to the correlation of the green signal on the red signal, Manders M2 is red on green); scale bar = 20 μm. All error bars represent 1 SD.