Analysis of differentially expressed mobilized CD34⁺ cells genes by qRT-PCR. The expression of 5 genes (FLT3, HOXA9, PTGS2, CXCR4, and CTSL) in CD34⁺ cells from the 3 rhesus macaque mobilized by plerixafor (A), G-CSF (G), or plerixafor plus G-CSF (A+G) were analyzed by qRT-PCR. The expression of CXCR4 was greater in plerixafor and plerixafor plus G-CSF–mobilized CD34⁺ cells. The expression of CTSL was greater on G-CSF– and G-CSF plus plerixafor-mobilized CD34⁺ cells. FLT3 expression was greatest on plerixafor-mobilized CD34⁺ cells, and the expression of PTGS2 was greatest on plerixafor plus G-CSF–mobilized cells. The level of expression of HOXA9 by the 3 types of CD34⁺ cells was similar but much greater than peripheral blood leukocytes. The expression of the 5 genes in leukocytes that were not absorbed by anti-CD34 from the 9 mobilized PBSC concentrates (CD34⁻) are shown as a control.