Blockade of endothelial NF-κB signaling reduced endothelial EPCR shedding. (A-B) Western blot photographs comparing tissue (A, kidney) and plasma (B) levels of EPCR protein in same groups of WT-Con (WT-C), WT-LPS (WT-L), TG-Con (TG-C), and TG-LPS (TG-L) mice. Actin and IgG indicate membrane for EPCR blotting was reblotted with actin or IgG antibody. (C) Densitometry quantification of EPCR bands. Compared with WT-Con and TG-Con mice, WT-LPS mice showed a markedly reduced tissue level of EPCR, in parallel with a significantly elevated plasma level of EPCR, indicating EPCR shedding. TG-LPS mice abrogated the LPS-induced reduction in tissue EPCR level and elevation in plasma EPCR level. Data are mean ± SEM of 5 mice. *P < .001 compared with any other group. #P < .001 compared with the WT-LPS group.