Blockade of endothelial NF-κB signaling prevented LPS-induced TM down-regulation. (A-C) Western blot photographs showing levels of TM protein in heart (A), kidney (B), and liver (C) of WT-Con (WT-C), WT-LPS (WT-L), TG-Con (TG-C), and TG-LPS (TG-L) mice. Actin indicates membrane for TM blotting was reblotted with actin antibody. (D) Densitometry quantification of TM bands. Tissue level of TM protein was high in all 3 organs of WT-Con and TG-Con mice, greatly reduced in organs of WT-LPS mice, and restored in the 3 organs of TG-LPS mice. Data are mean ± SEM of 6 to 8 mice. *P < .001 compared with any other group. #P < .02 compared with the WT-LPS group.