Representative Z-series confocal microscopy pictures of the muscle and spleen of mice treated with WT BMCs. Transplanted, BMC-derived, GFP-positive cells are seen in green (A,D). F-actin intermediate filament staining by Bodipy-phalloidin is seen in red (B,E), and colocalization of both stains is shown in the last panels (C,F). Muscle (A-C). Nuclei stained by DAPI are blue. Some GFP-positive cells are interstitial, and some are differentiated or fused with muscle fibers as shown by the colocalization of GFP-positive with muscle fibers (C). Spleen (D-F). Most of the GFP-positive cells in the spleen are part of the reticuloendothelium as determined by colocalization with VWF (blue) and F-actin staining (F). Scale bars represent 10 μm (A-C: 60×/1.4 NA oil objective; D-F: 40×/1.3 NA oil objective).