Representative Z-series confocal microscopy pictures of WT BMC-treated kidneys to demonstrate cell phenotypes. Transplanted, BMC-derived, GFP-positive cells are seen in green. (A) F4/80-positive macrophages are stained in blue, and CD45-positive leukocyte lineage cells are stained in red. Few GFP-positive cells are macrophages (white, arrows). (B) Distal tubular staining by D biflorus agglutinin (red). Some GFP-positive cells are colocalized with distal tubular cells (yellow, arrows). (C) Proximal tubular staining by Lotus tetragonolobus lectin (red). Some GFP-positive cells are colocalized with proximal tubular cells (yellow, arrow). (D) F-actin intermediate filament staining by Bodipy-phalloidin (red). Some GFP-positive cells are found in the glomeruli. (E) Tubular basement membrane staining by Ricinus communis agglutinin I (red). Some GFP-positive cells colocalize with the basal membranes (arrow). (F) Endothelial cell staining with anti-VWF antibody (blue). Some GFP-positive cells are colocalized with endothelial cells (white, arrow). Scale bars represent 10 μm (60×/1.4 NA oil objective), except for panel B, in which scale bar represents 50 μm (20×/0.75 NA air objective).