Flt3⁺CD150^– preGMs express genes important in T and myeloid lineage development. Three subsets of CMPs, along with HSCs, LMPPs, and DN3 cells, were purified from B6 BM and thymus. RNA from each population was reverse transcribed and used for real-time qPCR. Shown is the relative expression of indicated genes in each population after normalized to 18S RNA. HSCs were sorted as Flt3^–CD150⁺ LSK cells and LMPPs as Flt3^hiCD150^– LSK cells from B6 BM. DN3 cells were sorted as Lin^–Kit^–CD25⁺ thymocytes. Error bars denote SEM. Results shown are from at least 3 independent experiments.