Figure 3
Flt3+CD150– preGMs are T lineage-potent, but inefficient T progenitors. (A) Flt3+CD150– preGMs or MPPs were sorted from WT B6 BM (CD45B6) and intrathymically transferred at 1000 cells per mouse into sublethally irradiated congenic recipients (CD45SJL). Recipient thymi were analyzed 3 weeks after transfer for donor chimerism at the DP stage. n = 4. (B) Graphic representations of donor chimerism after intrathymic transfer of MPPs or Flt3+CD150– preGMs. *P = .01. (C) Flt3+CD150– preGM (1000 cells), Flt3–CD150– preGMs (10 000 cells), and LMPPs (1000 Flt3hi LSK cells) were sorted from WT B6 BM (CD45B6) and intravenously transferred into sublethally irradiated congenic recipients (CD45SJL). BM of recipient mice was analyzed on day 18 after transfer for donor chimerism in the B cell lineage (CD19+B220+). n = 4-5. (D) Donor chimerism in the B cell lineage after intravenous transfer of LMPPs, Flt3+CD150– preGMs, and Flt3–CD150– preGMs. N.D. indicates not detected. (E) Thymi of the same recipient mice that received intratransfer of LMPPs, Flt3+CD150– preGMs, and Flt3–CD150– preGMs were analyzed for donor chimerism at the DP stage on day 18 after transfer. (F) Donor chimerism at the DP stage after intravenous transfer of LMPPs, Flt3+CD150– preGMs, and Flt3–CD150– preGMs. N.D. indicates not detected. *P = 2 × 10–6 and **P = .01. P values were determined by Student t test. Error bars denote SEM. Results shown are from 2 independent experiments.

Flt3+CD150 preGMs are T lineage-potent, but inefficient T progenitors. (A) Flt3+CD150 preGMs or MPPs were sorted from WT B6 BM (CD45B6) and intrathymically transferred at 1000 cells per mouse into sublethally irradiated congenic recipients (CD45SJL). Recipient thymi were analyzed 3 weeks after transfer for donor chimerism at the DP stage. n = 4. (B) Graphic representations of donor chimerism after intrathymic transfer of MPPs or Flt3+CD150 preGMs. *P = .01. (C) Flt3+CD150 preGM (1000 cells), Flt3CD150 preGMs (10 000 cells), and LMPPs (1000 Flt3hi LSK cells) were sorted from WT B6 BM (CD45B6) and intravenously transferred into sublethally irradiated congenic recipients (CD45SJL). BM of recipient mice was analyzed on day 18 after transfer for donor chimerism in the B cell lineage (CD19+B220+). n = 4-5. (D) Donor chimerism in the B cell lineage after intravenous transfer of LMPPs, Flt3+CD150 preGMs, and Flt3CD150 preGMs. N.D. indicates not detected. (E) Thymi of the same recipient mice that received intratransfer of LMPPs, Flt3+CD150 preGMs, and Flt3CD150 preGMs were analyzed for donor chimerism at the DP stage on day 18 after transfer. (F) Donor chimerism at the DP stage after intravenous transfer of LMPPs, Flt3+CD150 preGMs, and Flt3CD150 preGMs. N.D. indicates not detected. *P = 2 × 10–6 and **P = .01. P values were determined by Student t test. Error bars denote SEM. Results shown are from 2 independent experiments.

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