Characterization of Bmi1 transgenic zebrafish line. (A) Schematic representation of lmo2:LDL-Bmi1-EGFP transgenic construct. pA indicates SV40 polyadenylation site. (B) Expression of Bmi1-EGFP fusion protein and transcripts in the progeny of mating Tg(lmo2:LDL-Bmi1-EGFP) with Tg (lmo2:Cre) line. (C-H) time-course WISH analyses of scl in the Bmi1-expressing transgenic embryos at indicated developmental stages. Red arrows and arrowheads indicate the increased scl⁺ HSPCs along the PD at 36 hpf.