Increased levels of inflammatory mediators are produced by macrophages in cmo mice before the onset of overt disease. (A) Secretion of cytokines and chemokines by cultured BMM from wt and cmo mice (n ≥ 5). (B) LPS-stimulated inflammatory mediator release by BMM of wt and cmo mice (n ≥ 5). (C) Reconstitution of PSTPIP2 expression in cmo macrophages by retroviral transduction. The vertical line indicates a repositioned gel lane from the same blot. (D) Reconstitution of PSTPIP2 expression in immortalized cmo BMM restores normal LPS-stimulated production of IL-6 and MIP-1α. (E) Screening of cytokine and chemokine levels in mouse sera from asymptomatic (6-8 weeks of age, n = 10-27) and diseased (20 weeks of age, n = 8-11) cmo mice and wt control mice. Data ± SD. *P < .05, †P < .01, Student t test. No significant differences in serum levels or BMM secretion were found for the other inflammatory mediators tested (BLC, D30L, Ltaxin, Eotaxin-2, Fas ligand, Fractalkine, G-CSF, GM-CSF, M-CSF, IFN-γ, IL-2, IL-3, IL-4, IL-9, IL-10, IL-12p40p70, IL-12p70, IL-13, IL-17, I-TAC, KC, Leptin, LIX, Lymphotactin, MIG, MIP-1γ, RANTES, SDF-1, TCA-3, TECK, TIMP-1, TIMP-2, sTNFRI, and sTNFRII).