DC-derived cytokines enhance the activation of HJ1 T cells on stimulation with Pam₃Cys-treated hCD1Tg BMDC. HJ1 T cells were enriched from liver leukocytes isolated from HJ1Tg/hCD1Tg/Rag^−/− mice, and were co-cultured with Pam₃Cys-treated hCD1Tg BMDC. Anti–IL-12 mAb, anti–IL-18 mAb, both anti–IL-12 and anti–IL-18 together, or an isotype-matched control mAb were added to co-cultures. Bar graphs depict the mean ± SD for the amount of IFN-γ or IL-17A in culture supernatants. Statistical significance was evaluated by comparing each experimental group with the isotype control group. *P < .05; **P < .01. Data are representative of 3 independent assays.