PRC2 complex binds TXNIP promoter and TXNIP is repressed in AML. (A) ChIP assays were performed in MOLM-14 cells using Abs against Suz12 or a negative control IgG and analyzed by RQ-PCR amplifying a region of −250 to −121 relative to transcription start site of TXNIP promoter. The relative enrichments of PRC2 complex in the TXNIP promoter region were normalized to isotype controls. The experiments were conducted in triplicates (mean ± SD). (B) MOLM-14 cells treated with DMSO control or DZNep 2μM for 48 hours. ChIP assays were conducted using Ab against H3K27me3. The relative reductions were shown in mean ± SD of triplicate measurement. (C) Real-time qRT-PCR analysis of TXNIP expression in healthy controls, AML cell lines, and primary AML patient cells. The expression of TXNIP in TF-1 cells was set as 1 (baseline), which is the lowest among the 6 cell lines tested. The average of C_T value of TXNIP in TF-1 sample was 24 ± 0.5. The numbers in the brackets indicate the sample size.