Figure 2
Figure 2. DZNep decreased the protein expression of EZH2, Suz12, H3K27me3, and induced cleaved PARP in leukemia cells. (A) MOLM-14 and Kasumi-1 cells were incubated with 0.1% DMSO, DZNep 0.25μM, 0.5μM, and 0.1% DMSO, 0.5μM, 1μM, respectively, for 48 hours. (B) Primary AML cells were treated with DZNep 5μM for 48 hours. Cells were harvested, lysed, and subjected to immunoblot analysis with primary Abs indicated. β-actin was used as loading controls. Densitometric analysis was performed using Amersham Image Scanner with LabScan ImageQuant TL Software (Amersham Biosciences). The expression ratio of EZH2, Suz12, and H3K27me3 was calculated as the DZNep-treated samples relative to control samples after normalization with respective β-actin level. Arrows indicate EZH2; C, cleaved; and FL (full-length), PARP protein, respectively. (C) Effect of DZNep on DNA fragmentation in MOLM-14 and Kasumi-1 cells.

DZNep decreased the protein expression of EZH2, Suz12, H3K27me3, and induced cleaved PARP in leukemia cells. (A) MOLM-14 and Kasumi-1 cells were incubated with 0.1% DMSO, DZNep 0.25μM, 0.5μM, and 0.1% DMSO, 0.5μM, 1μM, respectively, for 48 hours. (B) Primary AML cells were treated with DZNep 5μM for 48 hours. Cells were harvested, lysed, and subjected to immunoblot analysis with primary Abs indicated. β-actin was used as loading controls. Densitometric analysis was performed using Amersham Image Scanner with LabScan ImageQuant TL Software (Amersham Biosciences). The expression ratio of EZH2, Suz12, and H3K27me3 was calculated as the DZNep-treated samples relative to control samples after normalization with respective β-actin level. Arrows indicate EZH2; C, cleaved; and FL (full-length), PARP protein, respectively. (C) Effect of DZNep on DNA fragmentation in MOLM-14 and Kasumi-1 cells.

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