CLEC-2–deficient platelets fail to form stable aggregates under flow. Whole blood from control or INU1-treated mice (8 μg/g, day 5) was perfused over a collagen-coated surface (0.2 mg/mL) at a shear rate of 1700s−1. (A) Platelet adhesion on collagen after 30 seconds, indicated as number of platelets per visual field. (B) Aggregate formation on collagen after 4-minute perfusion time under anticoagulated conditions. (Top) Representative phase-contrast images. Bar represents 100 μm. (Bottom) Mean surface coverage (left) and relative thrombus volume expressed as integrated fluorescence intensity (IFI) per square millimeter (right) ± SD of 6 mice per group; **P < .01. (C) Aggregate formation on collagen after 4-minute perfusion time under nonanticoagulated conditions. (Top) Representative phase-contrast images. Bar represents 100 μm. (Bottom) Mean surface coverage ± SD of 6 mice per group.
