INU1-Fab partially inhibits CLEC-2 function in vitro. (A) INU1-Fab (20 μg/mL) induces aggregation of washed control platelets only when cross-linked (20 μg/mL anti–rat IgG). (B-C) Washed control platelets were incubated with vehicle (black) or INU1-Fab (20 μg/mL, gray) for 5 minutes at 37°C and then stimulated with the indicated agonists under stirring conditions. Light transmission was recorded on a Fibrintimer 4-channel aggregometer. The results are representative of 4 individual measurements.