Rheb2 overexpression expanded phenotypic HSC (KSL) cells in vitro and increased cell cycling. (A) Representative FACS plots of KSL cells in cultures of Rheb2- and Mieg3-transduced mBM cells. (B) Tabulated data from 2 experiments, each done in duplicate 4 days after transduction (mean ± SEM). (C-D) Representative histograms showing cell cycling of Mieg3- and Rheb2-transduced KSL cells and tabulated data from duplicate cultures; mean ± SD. *P < .05. Rheb2 GFP⁺ fraction compared with Mieg3 GFP⁺ fraction in panel D.