Rheb2 overexpression enhanced IL-3 proliferation and mTOR signaling in a mouse hematopoietic progenitor cell line. (A) Proliferation of transduced 32D cells in response to delayed IL-3 addition (combined data from 2 independent experiments each done in triplicate; mean ± SEM). **P < .01. (B) Western blot of 32D cell lysates after delayed addition of IL-3 ± rapamycin. Total S6 protein and β-actin are shown as loading controls. Western blot is representative of 2 independent experiments.