Figure 2
![Figure 2. CalDAG-GEFI and P2Y12 synergize in Cvx-dependent activation of Rap1 and ERK-MAPK. Washed WT or CalDAG-GEFI−/− (knockout [KO]) platelets were stimulated in standard aggregometry with (A) 100 ng/mL (low dose [LD]) or (B-C) 500 ng/mL (high dose [HD]) Cvx. Platelets were activated in the presence (C) of 75 μM 2-MesAMP to inhibit signaling by P2Y12. Rap1-GTP and phospho-ERK were determined in samples withdrawn at the indicated time points. Total ERK1/2 levels (bottom panel) were determined as a loading control. Results are representative of 3 individual experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/114/12/10.1182_blood-2009-04-218768/4/zh89990941970002.jpeg?Expires=1724940880&Signature=Zdx436Xq-EELHAgUUxHM6WIdRP4Ew5rtz7sWAfBRbInuTXh0-NX1eIskDA88Fcn9DQwg6HKU-~au~~LuUrKr25xSWUxr0HAhLb4-dOUk7rEps79m7L65DWnoCxc8O4KM40Z0qq4Ub0s-GGSFbJmqXItWC5yxiPJXho2rkLzns-XGQd0Lyj62Feq9EMOsWOQaXM5h-2iCojnOAiaLpjIbwl22Haw3X~sMWThdj6csgahTapRAqWnFyKcADyleMmlDrFAnU6oa3jZFrJDBefRkE5XA9d7p9ZEixC3OjTr6Rf58HhDIk6m2BVWXyOLgEbUSugzNLBaczY4ZrYuJSgZx2g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
CalDAG-GEFI and P2Y12 synergize in Cvx-dependent activation of Rap1 and ERK-MAPK. Washed WT or CalDAG-GEFI−/− (knockout [KO]) platelets were stimulated in standard aggregometry with (A) 100 ng/mL (low dose [LD]) or (B-C) 500 ng/mL (high dose [HD]) Cvx. Platelets were activated in the presence (C) of 75 μM 2-MesAMP to inhibit signaling by P2Y12. Rap1-GTP and phospho-ERK were determined in samples withdrawn at the indicated time points. Total ERK1/2 levels (bottom panel) were determined as a loading control. Results are representative of 3 individual experiments.
