TWSG1 inhibit up-regulation of hepcidin expression by BMP2/4 in human hepatoma cell line. (A) Relative hepcidin expression as a dosed response to BMP2 (□) and BMP4 (■) are shown. Final BMP concentrations are on the x-axis, and relative hepcidin expression on the y-axis (0 ng/mL assigned a value of 1 for comparison). (B) TWSG1 dose response of relative hepcidin expression in human HuH-7 cells in cultures supplemented with 10 ng/mL BMP2 (—), 10 ng/mL BMP4 (---), or in the absence of added BMP (). Bars represent mean values with asterisks signifying P < .05.