Intrinsic LFA-1 adhesiveness, but not VLA-4 adhesiveness is diminished in Kindlin-3–null LAD-III EBV cells. (A) Loss of Kindlin-3, but not of CDGI, in LAD-III EBV cells derived from patient A. Lysates of control and LAD-III EBV lymphoblasts were immunoblotted with anti–Kindlin-3 Ab, anti-CDGI mAb, and anti–total ERK2 Ab. (Bi) FACS staining of β₂ and CXCR4 on EBV lymphoblasts. (Bii) Basal and CXCL12-stimulated (c) or Mg²⁺-EGTA–stimulated (Mg) induction of the 327C β₂ epitope in control and LAD-III EBV lymphoblasts. (C) Spontaneous LFA-1 adhesiveness to high density ICAM-1-Fc by control and LAD-III EBV lymphoblasts under low shear stress (0.5 dyn/cm²). Frequency of transient and firm attachments determined in 2 fields of view for each group of cells is depicted as mean values ± range. (D) Spontaneous attachments (transient, rolling, or arrest) of EBV cells interacting with the indicated densities of VCAM-1-Fc at a shear stress of 0.75 dyn/cm². The frequency and strength of all tethers were determined, as in panel C. Data shown in panels C and D are each representative of 3 experiments.