Mitochondrial clearance is impaired in Atg7^−/− reticulocytes. (A) Immunofluorescence and flow cytometry of reticulocyte-enriched blood cultured for 3 days in vitro from wild-type and Nix^−/− mice and Atg7^−/− transplant recipients. The cells are stained with MTR. The black line in the histograms corresponds to day 0, and the shaded area to day 3. The scale is indicated at the bottom. (B) Flow cytometry of blood from phlebotomized mice stained with MTR (top panels) or doubly stained with MTR and TO (bottom panels). Mice were phlebotomized for 4 days. Thereafter, a small amount of blood (10 μL) was withdrawn on days 0, 1, and 3 for analysis. Day 0 was the day after the final day of phlebotomy. Genotypes are on the left.