Stable expression of FANCA in EUFA867 lymphoblasts partially corrects the phenotype of this cell line. (A) EUFA867 lymphoblasts stably expressing wild-type FANCA are still hypersensitive to growth inhibition by mitomycin C. FANCA-deficient HSC72 and EUFA867 lymphoblasts were transduced with SF91-FANCA. Viable cells were measured with the Cell Titer 96 Proliferation Assay. The data represent the percentage growth compared with untreated cells and show 1 representative result of 3 independent experiments with standard deviations. (B) EUFA867 lymphoblasts stably expressing wild-type FANCA show melphalan-induced G2 arrest. (C) EUFA867 lymphoblasts stably expressing wild-type FANCA have reduced FANCD2 monoubiquitination. Cells were treated with either 2 mM HU or 240 nM MMC for 16 hours or left untreated. Total lysates were immunoblotted for FANCM, FANCD2, and FANCA.